	   SnapGene      ¥GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCgccaccatgtcgaagccccatagtgaagccgggactgccttcattcagacccagcagctgcacgcagccatggctgacacattcctggagcacatgtgccgcctggacattgattcaccacccatcacagcccggaacactggcatcatctgtaccattggcccagcttcccgatcagtggagacgttgaaggagatgattaagtctggaatgaatgtggctcgtctgaacttctctcatggaactcatgagtaccatgcggagaccatcaagaatgtgcgcacagccacggaaagctttgcttctgaccccatcctctaccggcccgttgctgtggctctagacactaaaggacctgagatccgaactgggctcatcaagggcagcggcactgcagaggtggagctgaagaagggagccactctcaaaatcacgctggataacgcctacatggaaaagtgtgacgagaacatcctgtggctggactacaagaacatctgcaaggtggtggaagtgggcagcaagatctacgtggatgatgggcttatttctctccaggtgaagcagaaaggtgccgacttcctggtgacggaggtggaaaatggtggctccttgggcagcaagaagggtgtgaaccttcctggggctgctgtggacttgcctgctgtgtcggagaaggacatccaggatctgaagtttggggtcgagcaggatgttgatatggtgtttgcgtcattcatccgcaaggcatctgatgtccatgaagttaggaaggtcctgggagagaagggaaagaacatcaagattatcagcaaaatcgagaatcatgagggggttcggaggtttgatgaaatcctggaggccagtgatgggatcatggtggctcgtggtgatctaggcattgagattcctgcagagaaggtcttccttgctcagaagatgatgattggacggtgcaaccgagctgggaagcctgtcatctgtgctactcagatgctggagagcatgatcaagaagccccgccccactcgggctgaaggcagtgatgtggccaatgcagtcctggatggagccgactgcatcatgctgtctggagaaacagccaaaggggactatcctctggaggctgtgcgcatgcagcacctgatagctcgtgaggctgaggcagccatgttccaccgcaagctgtttgaagaacttgtgcgagcctcaagtcactccacagacctcatggaagccatggccatgggcagcgtggaggcttcttataagtgtttagcagcagctttgatagttctgacggagtctggcaggtctgctcaccaggtggccagataccgcccacgtgcccccatcattgctgtgacccggaatccccagacagctcgtcaggcccacctgtaccgtggcatcttccctgtgctgtgcaaggacccagtccaggaggcctgggctgaggacgtggacctccgggtgaactttgccatgaatgttggcaaggcccgaggcttcttcaagaagggagatgtggtcattgtgctgaccggatggcgccctggctccggcttcaccaacaccatgcgtgttgttcctgtgccgGAATTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTAGTGAGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC  =K      #          #   '   #      *            "   !   8      !            /                     $               /                                  !                        !   1       "      6      (   3       '      ,            +      "                        )   $      '         "   "   3         !                        &   %                                    *   $                           
                           $   .      2         %   (   '               !   %   '      	                                       	                !                     '             $   )                  !   (      *                  !                                 	               $   .             2   %            +         -               0   (   &   &                     !   #      (      #            0   )      )      *               +      )         š(aäåù>luvDì¯ìnŒù{,hz#  š1›½ÙoÄC±ºlú–	'?ë�ËÐäQoÌú|yQ!À+ø · °bæe		w (ZGòBí�-Ò@{  Ø �"©Â)¬…P	T
ImmE¡àí°‰=Á%�i # 	 d -´¾püYeª7DôE²ìÿŒÒêŽ»H	—	–dpŽö|û"' +u–Èj£ú§»mvÿä	°
×
(^¾6M•Ì‚DvÍ‡¬2z¯=}ÁZáù	#  ‡œ [»TlÎ¯	>
"@ì£µ»Öò~—è+]p}_8Rm,yS ›2ÚÅˆ3KZû<	W	]	
¬}Ñ KWåR*  ±e4� Óàî÷x^Óf	
´
ñ

F¡¦[H�óiCŽ©RhâÓ E¿”x£Mbœò1GŽ»Ü ¸ mŽçï©)a�âMî9§ìjâ.c¢A| g c«>ÆŸ	Õ‰88¯.Yµ’p" ½ ‚³	þ	[��F¢á0ÓãûH‚- F_Å%‚O>
 j! � Ï@sÊ3#)ª�'´1†Â%	|	6>e­ƒî$ÊÔþ¨å8 • U6Q}í€õ„Ã*­
+‘Q	ˆ	»	Á	JRnY¡gyû7‹”Ö"3F±Ì>cŠ>Y@ÓÂv&[ Ù LEè¿Ö	K	�	
U
nü9?sê˜:îœ§¹k2‚�! e 2õâí )/�I	U	†		
R
Õ
 Y7=beq¹ù�æþEÒ# 
 ?qZf«wF³µÙ-9�ë˜	¯÷ü_Kû‰ n .µ¿Ÿ¼ì
—Ö}˜[¶«ÉAå y -šý8Egg�ÓZO�nr§yà'qœI”äA†ÖO/ © ±¾b=@¤ÖHZ ¨·;Mn›±Mw“	±
)i±ZÆÍäƒ¤vw3{c€ŸŽ~�Âƒâ3 YÉkÜû¼÷íØ
_N–aó5MÎˆ	°B[m
j� ¬ vz¶Û 	u	å	)7q…Eéuýú_ A,‰—y‘ÀPi	c¿Íu¿Œ­Îƒ>C 8 Ñ hh¼¿m—ô	PS†ºÏš	°	?
E
A^¶Ó'&˜þ^Ñ• Þ ˆ\s¯Ûï	zm~`C-T ð �kÉ/V!
#/UâíöÉ×,² 9ùSab$    ¡R²U!•*
g
¤©¼óA’›éaqÅ nzã8Jxz‹¯   r~§ú´þ\tñ[¸X	
B
~�~žº¡àÓSáô3ž ž ê ñüL		0
H
,±Xº ÷ œ3“ÛdÆ‰æ4Øü
6
q
­ 	Ò4æ*Þ š Yd)sÕ­Ønú=	^	«	
ª
¸F\`VôLŽøüOàM/ ²…W~ó5‚ž¡§¹ø1½ŒÔ#	[	�	ü	G¤§kª4K“ô’8Fê€fi;� f¼rÔï¸'øg	
P
¶\`òkKš’ÐÜãÔ ] _ Üp@y�Ô	ò
¢;=I‘0DI�ªÆ”   s ºá_|Vç1	6	¦	ß	u
Ÿ
µ
5j´çš�Ø>ÈS9WN£• p M
î
Šh]™¬L¼ÝÏ FŽÀë0ƒc²ó2­Ë†Í˜ L �·ÞNyl´•¤
N�Hˆ�£c * Ô Óì#Dw•=¡	xç a ç _ípy	Ö	
�
“½`:ç±¿¸üºìïT|Š† C ¹ ü 7£ªC¨Sk¦L¾ÚCd–£B À n�ÁÅ„]{ðIŸZ
�
‚ÀJ”íBÈ(ãë‘áÌ! 0…è:³Ù	ú*bŸ´ã2N¥ÄØï<J¥³×]ñÒ/a‘} h è®Bd�ïÉÕ9° uªýT·�uc	 	ô
?…Í;WÀû9ýZ	Áó# ¦ q…?æ¾Çè
ÖŠü/¶è¯H š¬ÉC	Ã
™RšÚeñg“q kŒ	¨'ÿ	D\�›ž‰É.1I€âl÷DK`PàaQ w Ï X'â_päüƒGCáI?k ¾ å 5ƒ‚ŠÒ	´£^.¯½Æ&~!Ê! u Å ¼´	ƒ�™¯ÉÑ?G‡¬¶ÔÖºj´õ2@ƒç•ÿˆå6‚1 ÐAt*Ûå$/«Ùi6µÆ2‡¤§Ãì	3±bfª®4ßAz„®ï·›„%(>óöŠ‘©@  { ‘ ™®ŒÑaB8	}	p‰¸Ñ€)´–¡Ëï>k˜çYØæð" > ¥ 4‘(‘Ü&	Þ
ã
7’uy½ÁÅ7i©Ø¼ºÑßzáæÿ±·6 ! Ëáÿ¾*÷	?ÛtÊÕ¬¥„6 k Ç VzÞîð:X…+.=i‰®ã,Fõ3	R	¨	d
;i8•±×#4?s‹Zy?Þ/AÂ–¾ÃäœÙ;ƒ † Z7~~¼QüŒÚGPd#žUwÅø( Y – ² Ê JR�KÍÒÄ’ª‰	Â	Sà7hz‘î=#{’œ?ž)ÁX…Ç'Ô3 3 R /œ�öH#Ë	¼	
Ko×Z¢‡"¶²Í¬Z¶#4TÔ÷6B�·—¿6T\)hP  ; ÁãÕá	
þó:@ë*-é†¼	 ;©)VsºUzƒ½Ží' H Ú “ç¦èÃGÀó5z€×p	‚
†
·
Åõo™¸ÈÚï�¨+¢vÖòÆ8— QÊ
b9q\L	l
w
¡
ýCItÁË,ˆ‘kélÿ¤, #M]kÇðö´'Féž£áé	‚	¶	V
”
…ËÓu‰ßëô 
IlNÜ†½ÙÜ3 ¶ ™n!B¹ãø•	S
t'YêFCÏ®zP$€÷ÿ?y dJSk¦;	G	V	

=
Ö
!c´º|ñ–çV†«7lxà f 3œÒßöªî=ÅÒ³
ð
�á8›®F+ � ” ¼ �&³	*0�	J	€	‡	5ZXd ¬8>frxú‚ËÒÿ^›¦¸Ò$NÓý=ä ,>1´8Ž6®ø¦x&ý @ZµÈ" « @µ?x�ÛG¶¿Ú:LšìO’	bh°Yt‹‚Œ‚üBi  Ý g±È.O…Ú™	î	)
Òø`yaÐŠ rÔ[¬ûm	
+}°[L r Û»Vo¦·œû	O
4j©_æ€™e:Ï8�“M” ) K o �¶Òë"Cv L
í
k³\‡¢¬ÊÎ…æ B û /À\¢²~
B×~æ¤\¥·�½ûÑÙàB‰� S¥pÈb½B	˜Q„™ÌÙÈü®ò ‹�&‰Œ˜³ÈÐ>†È^o]¹áiJ}Bßà‡`ÉP) z )Úþ­ØhÐ�£¦ö	2atˆ©¼Ðs¨³¹Ðy• å'=òõj«?¶×5$ … .y�›ÝJÌ9h"E©	hP†�°z�"(>�ÝS5�½¾Ä(g G "³Àç&ÂFp�Ôò4[yk
…
…»r‡Û*¡uy' Ž “ c A›ÞIŽ©¸<²÷:		”	<
²
éÎÑ7]š¥·
Ok<wãöþ ª ´>�°¾ÇN™N‘	
(
*a­ÑZ¥wŠ�´Ï Úê¤on¥[¡a›K
Pj˜²[åûd�¡ŽÍ„ˆñL“" F „ (xŽ²¿Ë×oœDx—s»…Ç]„ ²Ïx†4|¼ ñÃ_4" ÊmÝ½ö
'
`Or—º„6±Î~…\‰™¤¶³	j ^Kõ’3 } ÐãPlüÓÐ
LMN	
‚ƒ˜£´µíî±²Chiˆ‰[\]J\nÚÛèòóôkl‘ ~ H	ÑäZQýÔøÑ
Ù
ô­ÁÊNïƒŠ–ému Âˆî^
O¹bõÏ
ÍÑD¯p]oÜq! I{­ 
~ºåw{·þÃv	Ò
8r+†õ¤þ•æ‹Ä@ê` x–_Í
/¦®¶ËemÝþ&0û*nv  ¡ [æ	Ú
*.€È$ÂO`«„¶µ . ­ ÒRÕÜù	F2êvð—›Ë a�Ö_$ò�?é5^ ‹ í-z’ÁïÊ	ÀÃÎcj�4„ÎÝr >Ãð:‰Š˜Qj	PºåúvÀ�ö¡õpE°šDp« Bc_
dÊ‹ÓÐ®ÏÒqíš&  +J®/c»ë”hÀÿv
Ðt}ƒ±	P_Ô €ù‡¥6 ¦% § |'ix�½žàõQT“s	
.
@
F
Ó
·Çs™ö3_–ç]Œ©AHM Ò ƒæ¬|¸n˜»8w	›	9ÿ�JR–a 9 åi¦‡ÄáÐ	9Bnw¶¿2(',ÚÿwÅÄÒë @  ‹—‰l¹ °0{§×[·<Ð�ùÞÿ=w <y&]™%
Î
ç¢¯Ì|n³.üo ß ')ã$tð	—n¥§+Y á `Ü9mfaD'1U ¢ í ô o¤÷±ûB*Wò£	Û
å
$ÊØÝp˜P¬·õW0E ñ ÿ P]\lÊ"
”V+�É¨ã:úb…³- € ¹žÈ0ãî÷ %.�ÃÓŠTìc8[eˆ Ðö„0ç	÷
ü
Ú/TÿR-³!áa$¶ö  WVÖ3
à
r½�fk1¦¾ÈGëñùy* 	×à{Ó!6S±SÄÍÝ–Þï+
h
ª“«S¼û3Pwèe˜Ìg9™T<$  / N ® H9•³V“"x(	‰
Ð{Ã¥ôþœêrŸ‘!oœÛä.{°¹  ¢Þ}Oˆú 	ò	{ï™BbVnîÆ{õ ªKÉŒ  Ö C½˜×Æò\¹
<Ÿá¡ht*) bbuC
œ¢ÔoâÌ@6 s¨ûLµ]·åY	øf‘�»vJê_Ÿ ã ‘îÿãV`�­%Ý;Tó‚õ”ý4R $Àðk
	ù	²r5X…»ûÞ , ‰ Œ Ÿ î�
:
I
-ÁÏ«¿È^hëÏs ë :Õ%Fyôf{“MÂ1
dYk¦ŽF;W†
 ò.ýË	¿Äfz ÄGNÇ0,Ùr
À
»
wÃÓÁ5Üº'!.®¾ß¬ ø ;iÿŠý
7
!öq| ?�Ë4<ÜŠ‹ç5Rk	ÁQ�±›EÀ # ”ñe™J�¨fº
®Ææûzç÷¢+XFœéqŸ › Z—åd?Þ 	¬	¹]M�ù&¯\áÖFX3€ `Í©?£®ÏÇ	Í	
eŒÔWZý!ÓÁ+rîÆ * o	_	AaJÑc©u×š e¬CtÖÙû>	`
«
GËõgŠPÐN›§$ ¯0€¼¨Á'`°k„O	 &=HklñßAˆë<‹ÀÑ. c ,³†ûXt•6Zi‡¢ºù2þY‹ÍÑéu�\	¯
•«½5L”oÕõ�Ÿ*\{1<Y  d“Ÿí wž	¥¨`¦ë70g�ºÛX´‘¡á2 KTÎ?rÉ5P|ì2Dôƒ¾~ÁÕ$	{	²	º	ý	Q“—Õ!ú9Gb~,Ä�#j1Œ§ m ž
á,(.T	Q
Ô
ë
XNa¸ß—›“ ^ªÑäúˆÕN ¨ ½ayˆ<ÕYŸö°Lh	t	ÌptÅu4Lèbž�2^% 7 ï gj¾r–ó RU”¹® 
f
T]yá¨È&%@šl`ÄBI®( ™ é ö Xq}¦ù(’³ýð×sð·ùª	
/
A
G
©
·�ó�Ò÷)à2¹Ý�' \ ^ }¹òq�0?{¼U÷0	5	œ	¥	Þ	t
£3:<J’³7EéHJ‘ÅÇVÛ Ó Ý<	‰” ‚˜«K—b ` „Äoz9Hžx	Õ	Ø	Œ
�
³1Ã“ARIê¹îS•… ç­A™tåÂ
ç
ó
>:Vd¿úŽðÔfç~ÀG t Ä j§»~WÑ	C«-®³1C?æ”ä!   ¤ à.˜5`ÅÛ7	ë	Ý
ox·À3Úy(6šÞàÉî=—X°¤% 2 Q X ± É IÑï-<G}ˆâÊô2	§	:6!µÙr›xÁT›Æ:÷ÓO' : ÆæÉâ8èo	à	v
�
“
 
¶
ÄòH)kèMÛ �¼ÙTþ¼ñÅì– µ » ˜m¥%ï
s&XEwÑš­Mx	 =ú|%~xÇh A q ú ŒRº±N
}
3i¨ƒËØ^åúÐß  !Š€!¢1‹Ï\¸!=Ž<½Þf> E ƒ '�nš–èNÌ‡Îý GÁ‡&
]
Ï
°Í}„³‚‡•Ÿ®oIp z^Ì
¥-#£1”ªådÊµÜ´/ =�ì9^#äù €ô3o™  *‚ä.uOmµ12I¨¥ à (#%k˜$
æ–¡¤¦ ó þ ¸öO\Çû
Ù -SþÜo—‰ë²,/DZd M ßUÌÿñ	zÏ˜ŽR¥ÇUOmžíø�dÚfŸ˜; Õ â B¼íKa¶¬;: ngsü)( + ˆ 9Ô$ExeŒ
9
¾ªWyúEV	 ;M€y½è¿ _–>¢äÝ	If [b¨À*E2 b Í>qÈ’C
_Xz	%”nðÞa};+Œ¹W³‹X  ˜ è î õ p¥ø‘²ü`qò+K­¨
SŽàË?ÑÿaÃ‡1] [ ÃT¤	×	Ž
æ
²“U¾�’èïQé¸íU}F! £ Ã Ðî7}Fón	€
’
Ü
Ãñ~Ùªq™­²Àíý»ãðö ´ º Q¤¡%2WD‚Ê;eg D ‚  ]†Œm\
•¤",©äM�†”É´ÛH ò ý -TÆË—#
å,0– Tlìcež—¤.:C 8A»^L«:Ÿ©Z§û¿)D¼~¾ Â p�ÂÇÏ^|ñJ¬m	‘
�ßðm}Tî‹èìVâ\ � º… [
$19£ä!+/ž•€“d�G½9f' Á oÆƒ„Þï&'()*jkl|žRS%KÔÕÖ×ÉÊäåæç$U’Ö¼$%  Ÿ6ŸÞÉÇ
Üâï…øŸÁ&LÄØCÃË)n‡%í×ÂÍ&‰ Ì éÝû†ù Â SÅ´ðAƒ=v¦´¬îÓme’ ÍÑ…Ž	Ä	c.ÅÙØrò!%³Ã'$ �ÔÊ”´‹	è	z
ø
ý
Uãð Û0äO'ÄÎÙÜ/€âoˆ^&·Ø÷bÎ) 1† 7 ß÷Mé#1B;+	Ú	¤
È
æë+µU3¦”"MDbÄÌ*K0 ~Š  žCêªÓú¡|BÊì6>w§­nf W¯e×
4
o
D¸Tl�2ñò“ éƒ%	¾�íð˜HÖ:<±úïz¡ i Í Xá
Þóüs‡Ãg´Æ§µ¿É„!-µÔ ' ÒTÎJƒ	i
@†Î<”¬TQxþ¢Ù8ÍÞh&€´=! 
v«Øþ|¸Ô²NÅ—_�	È~Æ«XÏüZùf™:ë$u(( áUÎ7’›‚†Þvßrð¡	,
õ
ü/uù	4:‹[ó"žÂôRUK –g"TdÍÅ	dÁ½ÆÚ\¤¸%Vé’HsV
šÄ* 0 5 O ¯ 3:r†Õ–W”yçµ¿Èé	Š
é
þ
V1FL¦$�(Ýé’™0·å/ÙøÏ I!âø´#{
ù
Mq×Å0ã'�é° @•)	Œ	ä¡°ÜP‹ÏÚ�ý_Á"pºc|±º  % §1Ž�LËÍñÑ|ÄåõÿësDp‰ Ü¸sI 2ó£‰b	¾	,èýç4fCNWÖLhïÇ¡+uÊ ¡¡©àÏP›<hD	ó	š¶Vš#fòcÅoÍ¹ö^§«�! 8øß~ê$­û2CÊÛ	¥
Ä
É
Ùì§÷
•cEF|”jr!   T ‡N	,	™
¼
|ç S\›¤Ûð‰®+8r1L‹  × ™ØÃ«d	ŸŒ ÊëãPmËøx+ ¿*g  Ÿ*©ÇóDëºþ®	ã	]¢}JCu^®|R D¾l�
]
E€ÔLhˆ¢7Lai¨ñQá-bo ç(À	 
Žœ =ûé6Š/2Eâ=�Xí?EuÉZ	 x ™vfØfY…	
<€mqåÕpHãDJ…@ X°(ã`£¾„òÍAl�  Ð QD
.õ‘ý±â7w” � ccå×5
p
E_�¹Uß3ûóNß „¸&‹æ"	Z	Ó	ž
µ/‘ñj™×=¢	 €Mêgw/	±Ì ¿ æ 6ƒ^ìY
ù’ž¿¤¼_I°;¾Ç'²ÖKðë{`Ë t©ü¶„€	
îû
"  v Î ä 4‚š®&ˆµÕkôö¼þH5� =  ä½‹Aâ
‘°ÄÞ@­h×¶»ƒ ‰æ�ïƒ$ j Æ YW
c
»Ößí<"O‘«YµÀ".3öAW„•¶ã–Ø5S‚. P\j’ïõ¥	a¢àµ	„ÊÒôýtˆÞêóÿ	B,H·Ç¨ÀÊ(+U…Øjè£Õ7  ÑRjÄÑ	F	4WcŽŸ«³àB{ð•­ÊUy…ª#6üß> Ü +Ó0Ú7K„lÙ	í	g¯5Xsÿ?Y÷`‹�A ( J Ñ!BuU¶ÇA	ú	AÇ£†¤9¼Î7­Ø’�2 ÙÜæ%IÁ%¬Ïñ3ˆ¥¨	õ	j
„
g²Ç`‡¨Ï=…¯º¸hœÜRŸÚ&)ôßitx'ªµÈ% � ’ Ùé£w³Žš¯�Æ˜C`9	~	�	;
J
¬ÐY\Ðÿú»ð;ðv | ÏâOÒõ
K¸q¹�¬ÀÉô[—¢ìÌÐgZÙçñt -   w•¬ÿ}¹Â.Ç*µý_šÃ?l%) Š `ÕïbÉ	ÂÉŠÒ
Žiñ™è4ìª+ ¦ &b«ê“œ¥g�ß’à7s|‚r	Ï	
-
8v¾ÆÙøv
5�\GQŸÃL ? &;Šâ¸Ö8Z;l{ÏŒø²<X ì n£°úÏõAƒ)ñ¢	ä
ö
“œQÒà§âô#õV7‡- ÖGVz 58°Ý|”N‡’ÃwÝî'	2
ˆ
ß
qzÂîýejª0½»úçô-© ÈS¸ —âÅUä€÷eœÜ›u“ËIQ ó #¿jø	¾Ç]eg¥qê:… " ÊFh/þ+I§e¹
¿
ÀÅÂÛ¹&W -{›­ž «Ì¨>Î	Æ	Ì	@Y%‰ÕtÖW¦Å™0 S4O{ú1sY†ì&¯ýjŠÌèÀN	¹	®
ÿ
<j¼–Ôž)@ê[zŠÃ0¿"/Ú0àÐ( 6 l Wï‡Ö	;X•¯-¶S	©	e
ê
WxM§%oÄ$tŒÞ(ß�1¸ù­œ& ;ñ€/>szäGö	/	4	Ý	s
‹
Á
ˆ29c²ùÂ6@GÓHe—Äæ¿Ú„& 1 P W ° È ÅHÈß-—,;4ÚÉê	G xØ5LÝ»ß0BSå“š<–¯ ù <ù$|
rÎvä $}wÆ 8 Ë
ãø"“Ÿnž  õ["jþú
NÎØÿRý�ÛQÆ1÷Ùê± ‡ :Jãd‹µ
8
½HeVrx(ù Z — Ì=‘	6ES§
ÂR±’ï©>Œ|Ð‘*þ`Â² ³ @,KS‹Å–ŸYëÈ(»}! ž5ÝÎ«l	Æ
Ûáî#8Œ¢Ýi{Q$�Âm†#~œÕF»Á## Ë ÌLŒÓè"A“*	Š	�	Ã	y
£
Tåê“ÃÍÛ.@‚u«q ld² �‚©$	
n
òCÒ·{³ì— ,5; ( & •f‘šMI‚^qÌÇ}Å Îtø[£·$UYøŠ‘¡7GÝU�êQtJ $ 4 02�á÷KÌLp¯EK#èC˜À¹r#  S ¨òÎga	½	˜
»
Ø[£çüˆæö	­eÕ7Eq¸]¦*it Â)æýc	­	¿	â	ÿ‹è5KgêD‡<OWt]ð
ÈY{¾,  œ ˜PäeÖ„	-ô;^½Þ
°úMÞ„vŽ ë!	X
�
	.i°Õ	Ë0 < 3O[iô¤Š¡@ß
b
ºÕÝéòþAì+!N�ª'*X¿»×Viâ×G¢�4�î€) I Û  ØHÐQÐØ	@	E	ô	ƒ
fÆV_�ž§W¹ÉgÆÛž©"£6_sw¬×ŽÇ _”è¢ÎávÁôÈ	J·[þ	�óðºÂ3ï©) %m‰¢a¯ùª·é@¤¯Üœ6{�íq	Î	
‡
pÕí›P÷Î÷,¨óPÇ ÉªE§ÄH¦=iã	¸
¾
ö›šX$ˆdp,ÔP9�˜* V Rùë%:®ü3rË	.	M	¸	Ü	­
‡wbnøKF‰ÒÞ/GRd •›  ô9cÊ
ÍÍþâ÷u’mvØ +Ë!Dx
¢
¦
Å
Úéí"‹¨ÂÌ-ltêª}k" à]p`	m
—
BDJ–d:¥s  Nh(Oê üƒ	W
a
œ
ÔÜñŠJÝh¯¾ÔÝ	�+ $^lˆÈ®øDu¶×GÛ¥âì		?	½
~IU^�¦ìOö‡Œ5O2Oœ¨  U ó-	·	–
¬
†Ìè!vŠÌáömÝ.9Qs)  fgñò­÷µÚŸ¤êë	•
š
›
}HT]œ¥àõ‹ÚÛÜ,-ÑÒÓ4MNŒ  1Ê  ›GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                        *                                                      	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
            
               
                           	                                                                         	                                                                                                                                  &                                            	                                       %                  	                                                         
   	                     	                           5   $         	      
         	                           	                              	            	            	               0         $   !      $         +   •   _      !   q   n         !      `                                                                                                                                                                             *                        	                                                                                                                                                                                                                                                           
    
     
           	                         	                                                &                	             %      	                     
 	       	         5 $   	  
   	         	          	    	    	     0   $ !  $   + • _  ! q n   !  `   vä  x"  xJ  x   }.  ‚�  ŠÜ�\ôÎe$Pë!¤?	¸	†Ì.Ngðž°Vm		Ùf®–J ÙR„)Q¸=	º;)[›àMÇzšE=à¶¶ü—	–ûp¸¾ MÇçkƒÁå#,	;S�’¼×õp¸ë:@8Œ£.4©Íüˆ´K½öz{ô	}ªË„ú		óMšú		˜	ì
óú		óóšó¤b|ñ
ø Ÿ¸	Vž©>—‰ªüuÁi�	J	«*
ÿ	«º •*
©’› NÁ„Z

 ,R	² ,R	¤vJÏŒª;	S
ùüÎU¤ž¡¦un¯øPûÊD	NøPûÊD	ª;	—#
å J	rr§ú´ž¡»«äJÃß”æÊ”à³	´
>FiŽÔ
ÕªXàIOé^¾~Õ 
é—9íŒ~ å	Á;X¯Ät�²är§ú´³	FS ž‹ã Žø X	 -šý´rÈN£ È£©ƒ	
ŠT
ÿ \+¨ã³uð~ çT
jÈ£¾~Õ— -šT
Im°$—; ³	F)?7u½Øy_S”f
¨
?	5j-4ê	ˆx—Vù³	ÈÐF‚ ±Ê” DàÒ<	³	»	T
´
)>Y¡FiŽ¬ÓÔ2[.|úýŠèzÎe]þ^Îøtwq«KK¶äeuaéÄ%®{�!	M	„	·	¬
U�;uŠê÷‰.ß¬	f\ï+)>ñôåþ†;VXÔb 
é×G^¦i;i?¾ß 
é7ßwZaé{�÷Í  -šýçT
Imj´r°ÈN£›Þ,
[KŽaé{�u÷¤vw Mz~á9BX‚
37ÅÓþbuª½Û•p²¸ÔØ;V½ÚG�ÂïvaéÄ{�!	÷7u½ØŽK  ±T£òèpmyyŠÏ üVß
qe½µ‘F+Û¹­Š…[
€“G[Hó£èýÖh¡-šï	ú;XýŠ¯èÄtz�Îe]²Ù¥`¨&ô,;šå	w¿âç ¡ŽË ç À1[	g	b™ªÈ­£¡  Í Nr›²øÅ×Þ—ç%�	,
V«X”�ð(T®ÏÊwý_Á[^K  ã La B“þ;VÜ%b
g¯Üâ×�:ƒ
-4ê	xÜg¯Ü¡‹^
Îøþt*�š \øÓ6n) Ò"Cvæ5|¨b¦¡ø  T£»òèpmy ¡AaÅzJ‹`KŽ	Ëa'1]RùÐë%Q®üËÐE	M	¸	­
V�žÉ‰©"/¦HqZ«¯KÇ ÛÞå$«+2‡¤§±„®×4·›Þ/Aä%(ó;©Ø LEè«	<	]	�	T
U
mK2�’pÂ(üc	Š‹JêO	¾eÓîf	
[âÓÍÅ	sVÄ«<	]	K’pÛå$«2‡¤§±„®·›%(ó©Ç Þ+×4Þ/Aä;¯Üí‡
pí,¨Ç¿	Ï°	^Ó<át-; Ç ÛÞå$'«+2‡¤§ÿ	
�›±@ë*„‰®×.14 €©·)›Þ/ADºUä%(ó;Ž©íRÂù(Ðë%Q®üËÐüE	M	c	¸	­
V�žŠ‹JêÉO‰©"/	¾4‘Ü&	Þ
yÁi©¼ºæÿ·8 h†A'&þÑN0»
Ó!.ßI  @	Æ£6¬×Ž»lÎ¯	>
@µ~—]Ý|N‡îô©È& I‚¡7Ýl 	-S	ê
WMÞùš1ÙÄúÐäQRšÚC½\ 	ù	
r
À
Á5¡h¯…»¾& l ³ 	I‚-S	ê
WMÞ¡7ÝùU¶ú	9Î7’F/+¿
ÂÛ¹& -­þSô3!Nh a
ÔÜñ¾žÉâl÷¬ “dÛ/¼ 	—­,²æ Eé*u�¹ßîó
 £5 žÒ†ÕCW”êJƒµé
V,L¡¦$|ÝBþL­0·u€øË!x
¢
éÂÌ-tªkb@H¨·;±“	±
Í~âR ¼	×Z¢‡¬Ô6·\)hü7DÒŽ7Žërïcøª¶Àò¨ó2¦¾	CÜ˜© =MM)¤v€@)	Œ	äÚ�pc±¼—“d­æ*rïcø¶Àò	Ü¬ Û/ 	,² Eéu; 'ÿ	
�›@ë*‰.1 €©)DºUŽíF/U+¶ú	¿
ÂÛ¹& -9Î7’­§ xžõs	s3çŒÍ….ò! Æò¹Êv Î ä 4‚š®&ˆµÕkôö¼þH5�< 3O[iô¤Š¡@ß
b
ºÕÝéòþAì+!N�ª'*X¿»×Viâ×G¢�4�î€kŒ	¨'ÿ	D\�›ž‰É.1I€âl÷DK`PàaQ* Ô Óì#Dw•=¡	xç… .y�›ÝJÌ9h"E©	hP†�°z�"(>�ÝS5�½¾Ä(gu Å ¼´	ƒ�™¯ÉÑ?G‡¬¶ÔÖºj´õ2@ƒç•ÿˆå6‚ù <ù$|
rÎvä $}wÆ   ¡R²U!•*
g
¤©¼óA’›éaqÅ nzã8Jxz‹¯Ù LEè¿Ö	K	�	
U
nü9?sê˜:îœ§¹k2‚�Â)æýc	­	¿	â	ÿ‹è5KgêD‡<OWt]ð
ÈY{¾,¦ &b«ê“œ¥g�ß’à7s|‚r	Ï	
-
8v¾ÆÙøv
5�\GQŸÃLG … • Ù "LU.6Qy}�›³ÀÝçí&EJ€ÂÌèõ9F„Ã*hp�­¿Ôò
"+4E[y‘©Ö		K	Q	�	ˆ	»	Á	
U
k
…
JRhnnüY¡9?Pgsy†�êû7‹”˜°Ö"3F…±»Ì:>czŠ>Y�"(>r‡�Ýî@Sœ§¹Ó5�½ÂÛ*kv¡¾&2[uy‚Ä(g� ? ¦ à &(*;‚Šâä&.b#%k«¸ê“œ¥gu�ß’à7s|‚˜r	Ï	
$
-
8Oæmvµ¾Ö1–Æ8ÙøZ2v¡
5;l{�ÏŒI\¤¦¨øGQŸ¥²Ã<LXs¨ûLµ]·åY	øf‘�»vJê_Ÿ  ¤ à.˜5`ÅÛ7	ë	Ý
ox·À3Úy(6šÞàÉî=—X°¤I V Û R ÂØù)HæÐë%:Q®ü3rËÐØý		.	@	E	M	c	­	¸	¿	Ü	â	ô	ƒ
­
f‡ÆÿwV_�ž§W‹è5KbgnêøDK‡¹É<OWgFt‰ÆÒÛž©Þ"/GR]dð
£È 6Y_sw{•¬¾×,ŽÇ›S4O{Âú)1sæY†Ñì&Rj¯ýjŠÌèÀÄÑý	F	N	c	­	¹	¿	â	®
ÿ
4<jÿ¼WcŽŸ«‹³àè5BKg{–ÔêðD‡•ž­Ê)<@UêOW[ztŠy…ªÃ#0]¿ð
"/6ÈÚ0Y{¾ü,àÐß> ¡ [æ	Ú
*.€È$ÂO`«„¶µà]p`	m
—
BDJ–d:¥se 2õâí )/�I	U	†		
R
Õ
 Y7=beq¹ù�æþEÒ#D ‚  ]†Œm\
•¤",©äM�†”É´ÛHf … s¨û*Lµ.y�›ÝJ]Ì39œ·Òßö ªî=h"Eo©ÅÒå		Y	_	³
ð
høAf‘��P†�áa°»Jvz8�"(>�ÑÝS5c�›®½F©¾Ju×Äê(g_šŸW¯e×
4
o
D¸Tl�2ñò“¢  'Ë       ž        ž¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
C*Œã¬Æ'Ñ™Ì¡Úhy)¥’[Zpi+dý™�+Ì§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDîàåäEýã§ƒ¦ÅigÞdSƒ“*W¥:	ñ;ö,Sý’ð>¶´M8ÜÓØ8wkàMØ�>hk}ý·OÁwƒm¸Ÿh?N¿HÊ†”¸ ì¸^»¶í„Ÿc}µ#7OpTê:áÿo¹ðü£ˆ;‘ÞT„o€íµL7��8Q@O{µ[s�—>vR!Ä]¾ àyÔ]¸Ô �H5œ�†¦®ïBÑP�¾pBX ï 4ÔP"ëà8�èøâ,AÛÐn¨ÄÐb†`ŠŒ¶¶óPÛ†/aJABøEQvÔi´ÕlE¶°B¡…o�H``ƒ²nChF¾4E¯£´Q†×Äàø¶â9íàDØmE?Ï÷°8PÒƒÖ4¾ñãmŽ.WOTæû�#64ß†NÈC„4ö+ß58¨Qz#íK{Ý¶=ƒØ2:é”Š“M:†JáÁ.ŽßBF|ýÈÿÛhâÁoôtì´?~'È6ëøŽ#PKÐCà8AÈ¼^£ØX Ô?ë­E‚Ú#‡Áƒ?ƒ¼:ß]ö2(¯ˆðZãFoMÒ<ìAçT}‡i•aÛuaÔ’?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~  ¦  	Þ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ¶<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4940-4956" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5009-5030" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4964-4970" color="#ffffff" type="standard"/><Segment range="4971-4988" color="#ffffff" type="standard"/><Segment name="-35" range="4989-4994" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2632" consecutiveTranslationNumbering="1" maxRunOn="2635" maxFusedRunOn="2635" detectionMode="exactProteinMatch"><Segment range="2567-2632" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6935-7039" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4746-4867" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3562-3697" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2670-2894" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3382-3711" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2940-3368" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5318-5903" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="2" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3778-4572" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6865" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6074-6865" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6866-6934" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="PKM2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="935-2527" color="#ccffcc" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>1bea3716-febc-41a6-aa8e-81b4d11f74a6</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="9:19:5">2019.8.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  &      p  v         v¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
C*Œã¬Æ'Ñ™Ì¡Úhy)¥’[Zpi+dý™�+Ì§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDîàåäEýã§ƒ¦ÅigÞdSƒ“*W¥:	ñ;÷’ôˆ ZãFoMÒ<ìAçT}‡i•aÛuaÔ’?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  ‡<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5289" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4498-5289" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5290-5358" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="Neo" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2202-2996" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3742-4327" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1364-1792" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1806-2135" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1094-1318" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1986-2121" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3170-3291" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5359-5463" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1056" consecutiveTranslationNumbering="1" maxRunOn="1059" maxFusedRunOn="1059" detectionMode="exactProteinMatch"><Segment range="991-1056" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="3388-3394" color="#ffffff" type="standard"/><Segment range="3395-3412" color="#ffffff" type="standard"/><Segment name="-35" range="3413-3418" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="3433-3454" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="3364-3380" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="MCS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="895-990" color="#ffff99" type="standard"/></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>4ff05e09-b66c-469c-8065-75f8cff75ce8</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="9:17:12">2019.8.21</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ¬ý7zXZ  æÖ´F !   t/å£à/Fl] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷ÀnÉ;_x’Í]¹õû’:·´{SÖ«ÃKÌµÛ	¹­O¼(Íaj�ÎÈ+ü…U¼]K5õÌÛó]>‹ wLÝÒõ8ƒT,n!Xq úE ³±b²Q²£“¶m1Tã½§‹ûg;)ÑÑ§å¢üý²@üJö°°J™)_å`—~„uNÁuEáå�|$é¦Ó}üeX1rì~_Þªy‹Ô[�¶]’‡šlý…!+ŒŽ|ŠåSŒŽ%#i]3¤û—Ë#$ü†ÀN`<oèpzu’Q�w¤©^íò7Òïm,óíŠ›ìwç4Œì¢gYÏW’"f-½óT�žx1ÙÖð•?añ¯–û›Ù®¶pÖ=�#ýÅÑ€MS××jeÚg;t�†sóqÆA/—B¢FÇÎzÚÜB¨ì°tµÚè:B-N�¿ËG�Âµ.ßõl�–À:§#’BXúäó~ÄTw(äcd˜ãC—+(XàøoÜ¼©òÐw;•-S½œ�Û¥rÁ}O3ËÛFSoT…Rü~]HkÿÕ½oj‘UÎ{‹ÚdbŽ5Y€Ü'ZË;j>C…ñh’{€{%ëZV‘·¤vxüð;´iR^©v£w@·<�ƒüidA­O«*ìu³\8Ä/Ÿl|½æ›àõšðH¢Žù”Q„Bþ¾ÛLq©“Í_«l#P& (Tê'R"£öÅjÎ(Šð£ªìæ\x°bÊ‡Ü¼¶â‚=1VcLéíK!é|3ó‚!¼·�ºÓé„4:±¦å±Ò¥uLò€î±mçxH·Ïî"g[ì	4EåR0ÉK®*V*_[ov¹oîÉË´äp»ìÍZ%®ð¨èi÷N¸ÈMH/f9 ]%Ü¼�)µñ€YZsUÊË…™\±âj�,0°Â"¤ oÃ®¸”ñ4Ûb„ÚXßO;zœ™Ð5i,ÄàdÃûbÑå!&K n`äèG¼…eLpy«èiFÖ³„WÏèüÚ‚-Ÿ¼Z=¨�p¦vF h
®mtÿy²ßQvkvbZ¨äi¹…6]´!Ët/.TLÍ”¦<N(l'EÅb�–%	3#¦ÅÉ
ƒ/®‘Úu-1ÀYOlâJÛo™MuÏÃÓáBOc{êeNÈ&ž3R©Î–�ÎJ; ‘y'ŽçöÎE<ø$þË³R!I²³[tëâª1ûž)O’¤@Z…aZ‰ùélö“�‘�þ³öî�å@R=T$YOÕ·ç˜_¶ˆÇ¦o¼ ÀD¬| TvªÓz6é=½w%P1Skwhtâºm¢„r¥lvÉÎ~ÛF¨ùÆ@êãD+Q�e0uÔÁ7âÐf3ÞÒ–ö™À2²'o�àág¦)Ú½”SºfZ'à‚ín ÁD€P{ÇÕ4œÙ5&ƒÚïÀ1‰à†ÃgÃøbroOì=³“]˜±î}7Q“=Ž*YFYUæŠ�ÎåÔ}¥ÙI»Ó}¨ƒ½`§v{v3€€öÏOÂÛ¹7ï"Ô´®ä{z5ÉÑ—Ä°½¢µ'oUõØÓmq¯&^Ý9‚ŽÔ‘‹ú5.núrÞT¿giƒ­èžÀæëCIƒbTø¿DÜÒ&ò‚×ûõ*Î¾O Ú“xÐd¢„¨©¸TdãÅWª/Ñ7”ä’6ÙT4§é¨ë4RÉÅœb]tUÆÆ–¾ï?…÷‚¯J,àð®ã‘ƒ‘d=³b¦�Ó·||Íre »£-Òà%=uüìÒÚ"âœ†-Â«O¶qÇdþÑ-Ãg–v±&sÜKûmêu+€‡ÔÍ�Ì‰în³7î3™ã,Å‹ºœ®¬;!€Wá»dC ÿÎö1Õýò¼ÕTA±ö/âè8›¡�~‡¡÷�’Ú:¹§‘¨‚»pMÄð†ÓÑ?u›l8^3"¿¤,n9)±& C�8“KTíÜ
H�±(•ý|Ù0£›ƒgéÅ]Þ<ÜZ)Î·‘�1 í>p:À¹À(ë\1›‡}!¼ ï‰œkš<�³´mŸô�I \�ùC³[Œ1Ñ@/Ê= Ýž‘1„’:®öt\tQ´•VgJ¥Ð¨œÕqS
úª½ÜÔbLŒ£ÿIeH¿ÿñOì·¦Ý&aÏ§ªX”…ßŽ½÷#«2Æ0]	n¼{é6ÖÎ¿©ØŠ)¸Ž‘©öhGúŠôyU™÷\�P%7‘·nÄD±ëC¾?‚KËB]f�s#L£9=E�ì›Û
'†‚eÙW÷);m«�z¦¹z÷lÙÏÉ¯êÏweŸ£ùÑ¼R‰ƒaÄì•!hÊ7I�è±´óƒ˜�…€Vh.¬ªCÇm}Å¨äf�AH®}äfdÁþ[–G"¢dSÒžì¿Ø4Ã?ê6z§eæP[D�ðð�·Ð”ž•­KÄæ›…Âõnu%   Ÿ=‚Áv} ˆÇ^  ¼ ^>±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
   g<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="0" name="PKM2" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="941-2533" color="#ffcc00" type="standard"/></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6871" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="6080-6871" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6872-6940" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="2" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3784-4578" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5324-5909" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2946-3374" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3388-3717" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2676-2900" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3568-3703" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4752-4873" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6941-7045" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2638" consecutiveTranslationNumbering="1" maxRunOn="2641" maxFusedRunOn="2641" detectionMode="exactProteinMatch"><Segment range="2573-2638" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDHDGDYKDHDIDYKDDDDK"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4970-4976" color="#ffffff" type="standard"/><Segment range="4977-4994" color="#ffffff" type="standard"/><Segment name="-35" range="4995-5000" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="5015-5036" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4946-4962" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¤<Notes>
<UUID>b593a324-8d34-4810-b811-d33092cf3ccf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-PKM(human)-3Ã—FLAG-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="4:13:3">2025.8.27</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      HO  d               E              ÿÿÿÿYô? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
